PARP Activity Assay Service

Poly (ADP-ribose) polymerases (PARPs) are a family of enzymes that play a pivotal role in cellular functions, particularly in DNA repair, genomic stability, and cellular signaling. The development of robust assays for PARP activity is critical for drug discovery and evaluation of PARP inhibitors. Alfa Cytology offers comprehensive PARP activity assay services for our clients.

Introduction to PARP Activity Assay

The most studied member, PARP1, is integral to the cellular response to DNA damage, utilizing nicotinamide adenine dinucleotide (NAD⁺) to catalyze the addition of ADP-ribose polymers to target proteins. This post-translational modification is crucial for recruiting DNA repair factors to sites of damage, thus facilitating effective repair mechanisms. Given the significant role of PARPs in cancer biology and therapeutic resistance, the development of robust PARP activity assays is essential for drug discovery and the evaluation of PARP inhibitors.

Fig. 1 Principle of PARP activity assay. (Wigle T. J., et al. 2020)

PARP activity assays are essential tools for measuring the enzymatic function of PARPs in various biological samples. These assays provide insights into the status of DNA repair mechanisms and help identify alterations in PARP activity associated with different cancer types. By assessing PARP activity, researchers can elucidate mechanisms of drug resistance, evaluate therapeutic efficacy, and discover novel biomarkers for patient stratification.

Our Services

At Alfa Cytology, we specialize in providing comprehensive PARP activity assay services tailored to meet the needs of preclinical research. Our advanced assay platforms incorporate both in vitro and cellular models, employing cutting-edge technologies such as time-resolved förster resonance energy transfer (TR-FRET) and bioluminescence resonance energy transfer (NanoBRET).

Enzyme Activity Measurement

We utilize advanced bioluminescent assays, such as the NAD/NADH-Glo assay, to measure PARP activity accurately.

High-Throughput Screening

Our high-throughput screening services facilitate the simultaneous evaluation of multiple compounds against PARP targets.

Selectivity Profiling

Utilizing techniques such as BRET technology, we provide insights into binding affinities and residence times, crucial for understanding the therapeutic window of PARP inhibitors.

Custom PARP Assay Kit Development

For researchers interested in developing custom PARP biochemical assay kits tailored to their specific needs, Alfa Cytology is here to assist.

Cell Viability Assays

In conjunction with PARP activity assays, we provide cell health assays to evaluate the cytotoxic effects of PARP inhibitors on cellular viability.

Drug Combination Studies

We facilitate the evaluation of drug combinations involving PARP inhibitors and other therapeutic agents.

For researchers seeking to advance their understanding of PARP biology and explore comprehensive PARP activity assay services, Alfa Cytology is ready to assist. Our dedicated team of experts is committed to providing tailored support, from initial consultation to the delivery of detailed assay results. To learn more about our services or to discuss your specific research needs, please contact us.

Reference

Wigle T. J., Blackwell D. J., and et al. In Vitro and Cellular Probes to Study PARP Enzyme Target Engagement. Cell Chem Biol. 2020, 27(7): 877-887.

For research use only. Not intended for any clinical use.